O papel da fibrina rica em plaquetas e leucócitos na cicatrização óssea de defeitos em calvária: estudo em coelhos
Conteúdo do artigo principal
Resumo
Introdução: O L-PRF é concentrado de plaquetas e leucócitos em rede de fibrina, obtido pela centrifugação autóloga coletada no momento da cirurgia. Faz parte da segunda geração de concentrados plaquetários, sendo de baixo custo, fácil preparo, simples obtenção e tem a capacidade de acelerar a cicatrização de tecidos moles e duros.
Objetivo: Revisar o reparo ósseo de defeitos não críticos em calvária utilizando o L-PRF isoladamente e em associação com osso autógeno particulado.
Método: Revisão integrativa da literatura retirada das plataformas SciELO, Pubmed e Scopus em inglês e português utilizando os seguintes descritores: “L-PRF, cicatrização óssea, defeitos em calvária. Coelhos” em AND ou OR pelo título ou resumo. Após a seleção apenas os artigos cosiderados pertinentes foram lidos na íntegra.
Resultados: Foram incluídos 39 artigos.
Conclusão: O L-PRF isoladamente teve efeito positivo na formação óssea no decorrer das semanas.
Detalhes do artigo

Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Referências
Choukroun J, Adda F, Schoeffler C, Vervelle A. Une opportunité en paro-implantologie: le PRF. Implantodontie. 2001;4:55-62.
Dohan MD, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101(3):37-44. Doi: 10.1016/j.tripleo.2005.07.008
Ehrenfest DMD, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol. 2009;27(3):158-67. Doi: 10.1016/j.tibtech.2008.11.009
Lauritano D, Avantaggiato A, Candotto V, Zollino I, Carinci F. Is platelet-rich fibrin really useful in oral and maxillofacial surgery? Lights and shadows of this new technique. Ann Oral Maxillofac Surg. 2013;1(3):1-5. Doi: 10.13172/2052-7837-1-3-826
Borie E, Oliví DG, Orsi IA, Garlet K, Weber B, Beltrán V, Fuentes R. Platelet-rich fibrin application in dentistry: a literature review. Int J Clin Exp Med. 2015;8(5):7922-9.
Miron RJ, Choukroun J. Platelet rich fibrin in regenerative dentistry: biological background and clinical indications. John Wiley & Sons, Ltd. 2017. Doi: 10.1002/9781119406792
Zhao QM, Ding YJ, Si T. Doi: Platelet-rich fibrin in plastic surgery. OA Evidence-Based Medicine. 2013;1(1):1-6. 10.13172/2053-2636-1-1-512
Dohan MD, Choukroun J, Diss A, Dohan SL, Dohan AJJ, Mouhyi J et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part II: Platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101(3):45-50. Doi: 10.1016/j.tripleo.2005.07.009
Mallmann F, Lago PEW, Bona ADU. Uso de fibrina rica em plaquetas (PRF) no tratamento de perfurações da membrana sinusal. Full Dent Sci. 2013;5(17):59-66.
Blinstein B, Bojarskas SE. Efficacy of autologous platelet rich fibrin in bone augmentation and bone regeneration at extraction socket. Stomatologija. 2018;20(4):111-18.
Blatt S, Thiem DGE, Pabst A, Al-Nawas B, Kämmerer PW. Does platelet-rich fibrin enhance the early angiogenetic potential of different bone substitute materials? An in vitro and in vivo analysis. Biomedicines. 2021;9(1). Doi: 10.3390/biomedicines9010061
Eshghpour M, Dastmalchi P, Nekooei AH, Nejat A. Effect of platelet-rich fibrin on frequency of alveolar osteitis following mandibular third molar surgery: a double-blinded randomized clinical trial. J Oral Maxillofac Surg. 2014;72(8):1463-7. Doi: 10.1016/j.joms.2014.03.029
Marenzi G, Riccitiello F, Tia M, di Lauro A, Sammartino G. Influence of leukocyte- and platelet- rich fibrin (L-PRF) in the healing of simple postextraction sockets: a split-mouth study. Biomed Res Int. 2015:1-6. Doi: 10.1155/2015/369273
Kumar YR, Mohanty S, Verma M, Kaur RR, Bhatia P, Kumar VR, Chaudhary Z. Platelet-rich fibrin: the benefits. Br J Oral Maxillofac Surg. 2016;54(1):57-61. Doi: 10.1016/j.bjoms.2015.10.015
Canellas JVS, Ritto FG, Medeiros PJD. Evaluation of postoperative complications after mandibular third molar surgery with the use of platelet-rich fibrin: a systematic review and meta-analysis. J Oral Maxillofac Surg. 2017;46(9):1138-46. Doi: 10.1016/j.ijom.2017.04.006
Diana C, Mohanty S, Chaudhary Z, Kumari S, Dabas J, Bodh R. Does platelet-rich fibrin have a role in osseointegration of immediate implants? A randomized, single-blind, controlled clinical trial. J Oral Maxillofac Surg. 2018;47(9):1178-88. Doi: 10.1016/j.ijom.2018.01.001
Stähli A, Strauss FJ, Gruber R. The use of platelet-rich fibrin to enhance the outcomes of implant therapy: a systematic review. Clin Oral Implants Res. 2018;18(18):6-19. Doi: 10.1111/clr.13275
Zhou T, Yang HW, Tian ZW, Wang Y, Tang XS, Hu JZ. Effect of Choukroun platelet-rich fibrin combined with autologous micro-morselized bone on the repair of mandibular defects in rabbits. J Oral Maxillofac Surg. 2018;76(1):221-8. Doi: 10.1016/j.joms.2017.05.031
Oliveira LA, Almeida FLD. Liberação de VEGF, TGFb e FGFb da matriz de fibrina rica em plaquetas e leucócitos obtida pelo protocolo de centrifugação. Rev Catarinense de Implantodont. 2019;19:29-37.
Ehrenfest DMD, Pinto NR, Pereda A, Jiménez P, Corso MD, Kang BS, et al. The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte- and platelet-rich fibrin (L-PRF) clot and membrane. Part 1: evaluation of the vibration shocks of 4 models of table centrifuges for L-PRF. Poseido. 2014;2(2):129-139.
Pinto NR, Pereda A, Jiménez P, Del Corso M, Kang BS, Wang HL, et al. The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors and fibrin architecture of a leukocyte- and platelet-rich fibrin (L-PRF) clot and membrane. Part 2: macroscopic, photonic microscopy and scanning electron microscopy analysis of 4 kinds of L-PRF clots and membranes. Poseido. 2014;2(2):141-54.
Ehrenfest DMD, Pinto NR, Pereda A, Jiménez P, Corso MD, Kang BS, et al. The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte- and platelet-rich fibrin (L-PRF) clot and membrane. Platelets. 2018;29(2):171-84. Doi: 10.1080/09537104.2017.1293812
de Oliveira LA, Borges TK, Soares RO, Buzzi M, Kückelhaus SAS. Methodological variations affect the release of VEGF in vitro and fibrinolysis' time from platelet concentrates. PLoS ONE. 2020;15(10):e0239562. Doi: 10.1371/journal.pone.0240134
Kobayashi M, Kobayashi M, Kawase T, Horimizu M, Okuda K, Wolff LF, Yoshie H. A proposed protocol for the standardized preparation of PRF membranes for clinical use. Biologicals. 2012;40(5):323-9. Doi: Doi: 10.1016/j.biologicals.2012.07.004
Nelson EA, Keller GL, Mitchell TW, Pennypacker B, Rebbeck P, Rogers IT. A jugular bleeding technique in rabbits. Lab Anim. 2010;39(1):17-22. Doi: 10.1038/laban0110-17
Oliveira MR, de C Silva A, Ferreira S, Avelino CC, Garcia IR Jr, Mariano RC. Influence of the association between platelet-rich fibrin and bovine bone on bone regeneration. A histomorphometric study in the calvaria of rats. J Oral Maxillofac Surg. 2015;44(5):649-55. Doi: 10.1016/j.ijom.2014.12.005
Panda S, Ramamoorthi S, Jayakumar ND, Sankari M, Varghese SS. Platelet rich fibrin and alloplast in treatment of intrabony defect. J Pharm Bioallied Sci. 2014;6(2):127-131. Doi: 10.4103/0975-7406.129178
Acar AH, Yolcu Ü, Gül M, Keleş A, Erdem NF, Altundag Kahraman S. Micro-computed tomography and histomorphometric analysis of the effects of platelet-rich fibrin on bone regeneration in the rabbit calvarium. Arch Oral Biol. 2015;60(4):606-14. Doi: 10.1016/j.archoralbio.2014.09.017
Ortega-Mejia H, Estrugo-Devesa A, Saka-Herrán C, Ayuso-Montero R, López-López J, Velasco-Ortega E. Platelet-rich plasma in maxillary sinus augmentation: systematic review. Materials. 2020;13(3):736. Doi: 10.3390/ma13030622
Blatt S, Thiem DGE, Kyyak S, Pabst A, Al-Nawas B, Kämmerer PW. Possible implications for improved osteogenesis? The combination of platelet-rich fibrin with different bone substitute materials. Front Bioeng Biotechnol. 2021;16(9):680912. Doi: 10.3389/fbioe.2021.640053
Abdullah WA. Evaluation of bone regenerative capacity in rats calvarial bone defect using platelet rich fibrin with and without beta tricalcium phosphate bone graft material. Saudi Dent J. 2016;28(3):109-17. Doi: 10.1016/j.sdentj.2015.09.003
de Oliveira ES, Ribas-Filho JM, Sigwalt MF, Lourenço ES, Figueiredo FP, Czeczko NG, et al. Platelet-rich fibrin improves the osteoneogenesis in non-critical defects in calvaria: a histological and histometric study. Acta Cir Bras. 2023;38:e383423. Doi: 10.1590/acb383423
Lee JW, Kim SG, Kim JY, Lee YC, Choi JY, Dragos R, et al. Restoration of a peri-implant defect by platelet-rich fibrin. Oral Surg Oral Med Oral Pathol Oral Radiol. 2012;113(4):459-63. Doi: 10.1016/j.tripleo.2011.03.043
Pripatnanont P, Nuntanaranont T, Vongvatcharanon S, Phurisat K. The primacy of platelet-rich fibrin on bone regeneration of various grafts in rabbit's calvarial defects. J Craniomaxillofac Surg. 2013;41(8):649-55. Doi: 10.1016/j.jcms.2013.01.018
Durmuşlar MC, Ballı U, Öngöz Dede F, Bozkurt Doğan Ş, Mısır AF, Barış E, et al. Evaluation of the effects of platelet-rich fibrin on bone regeneration in diabetic rabbits. J Craniomaxillofac Surg. 2016;44(2):126-133. Doi: 10.1016/j.jcms.2015.11.009
Liu Y, Sun X, Yu J, Wang J, Zhai P, Chen S, et al. Platelet-rich fibrin as a bone graft material in oral and maxillofacial bone regeneration: classification and summary for better application. Biomed Res Int. 2019;2019:6387612. Doi: 10.1155/2019/3295756
Canellas JVDS, da Costa RC, Breves RC, de Oliveira GP, Figueredo CMDS, Fischer RG, et al. Tomographic and histomorphometric evaluation of socket healing after tooth extraction using leukocyte- and platelet-rich fibrin: A randomized, single blind, controlled clinical trial. J Craniomaxillofac Surg. 2020;48(1):24-32. Doi: 10.1016/j.jcms.2019.11.006
Awadeen MA, Al-Belasy FA, Ameen LE, Helal ME, Grawish ME. Early therapeutic effect of platelet-rich fibrin combined with allogeneic bone marrow-derived stem cells on rats' critical sized mandibular defects. World J Stem Cells. 2020;12(1):55-69. Doi: 10.4252/wjsc.v12.i1.55
Dragonas P, Katsaros T, Avila-Ortiz G, Chambrone L, Schiavo JH, Palaiologou A. Effects of leukocyte-platelet-rich fibrin (L-PRF) in different intraoral bone grafting procedures: a systematic review. Int J Oral Maxillofac Surg. 2019;48(2):250-262. Doi: 10.1016/j.ijom.2018.06.003
Fernandes F, Obregón PL. Características de vítimas de violência durante o período peri-pandêmico de COVID-19. BioSCIENCE. 2022;80(2):63-9. Doi: 10.55684/80.2.15